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	Provedor de dados Genet. Mol. Biol. (1) Autor Jolodar,Abbas (1) Miller,David J. (1) Palavra-chave Filarial parasite (1) Nematode (1) Salmonella typhimurium (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2002 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Construction and expression of aspartic protease from Onchocerca volvulus* as ompA fusion protein in a mutant strain of Salmonella typhimurium Genet. Mol. Biol. Jolodar,Abbas; Miller,David J.. Two constructions in pHS164 vector were designed to permit expression of OV7A and OV4A inserts encoding the N-terminal and C-terminal portion of an aspartic protease from Onchocerca volvulus, respectively. A novel 39 kD protein ompA-OV7A fusion protein was stably expressed as ompA fusion in a modified strain of Salmonella typhimurium strain SL5000 and E.coli strain JM109. Expression of the fusion protein in bacterial strains harboring the constructs were evaluated by western blotting. E.coli and Salmonella lysates were fractionated by 10% SDS-PAGE gel and then immobilized to nitrocellulose membrane by electroblotting. Primary polyclonal antibody generated in rats against the GST-OV7A fusion protein was used in the Western blots. It remains to be seen... Tipo: Info:eu-repo/semantics/article Palavras-chave: Filarial parasite; Nematode; Salmonella typhimurium. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100018 Registros recuperados: 1 Primeira ... 1 ... Última

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